Membrane Preperations and Assay Buffers

From Assay Guidance Wiki

Types of membrane preparations used

1. Crude homogenates (2)
2. Plasma membrane preparations (P2 fraction, 3)
3. Sucrose density gradient enriched receptors (1)
4. Commercially available membranes, which includes:
   • Receptors cloned into mammalian cells (Perkin Elmer, Euroscreen, Cerep)
   • Receptors cloned into Sƒ9 insect cells co-expressing mammalian G-proteins

Types of Assay buffers

1. Lazareno and Birdsall buffer (2) 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, pH 7.4.
2. Buffers with HEPES replaced by 50 mM Tris HCl (1,9)