NCGC Logo Link to HomeLink to About UsLink to Assay GuidanceLink to News Events & PublicationsLink to Contact UsLink to Resources
Assay Guidance  //  
Personal tools
Other Resources

Section11:Important Parameters for Development of an Immunoassay

From Assay Guidance Wiki

Jump to: navigation, search
  1. Primary or capture antibody
  2. Secondary or detection antibody
  3. Plate type
  4. Coating buffer
  5. Blocking buffer/diluent buffer
  6. Wash buffer
  7. Coating antibody concentration
  8. Coated antibody stability
  9. Timing of each step in the immunoassay
  10. Secondary antibody concentration
  11. Reporter concentration
  12. Readout
  13. Instrument linearity
Retrieved from "http://spotlite.nih.gov/assay/index.php/Section11:Important_Parameters_for_Development_of_an_Immunoassay"
Link to Health and Human ServicesLink to National Institutes of HealthLink to First Gov.com
Link to Site Map Link to Privacy Policy Link to Terms & Conditions
Copyright © 2011, National Institues of Health Chemical Genomics Center. All Rights Reserved.